dengue serotype 3 vlp  (Native Antigen Inc)

Journal: PLoS Neglected Tropical Diseases

Article Title: Analysis of Cross-Reactive Antibodies Recognizing the Fusion Loop of Envelope Protein and Correlation with Neutralizing Antibody Titers in Nicaraguan Dengue Cases

doi: 10.1371/journal.pntd.0002451

Figure Lengend Snippet: (A) Virion-capture ELISA was performed using serial dilutions of a human mAb 82.11 with known c oncentrations to generate a standard curve. (B) Sequential human serum samples from a dengue patient were tested simultaneously with the standard and the OD values were interpolated to determine [anti-E Abs]. Data are means with standard deviation of duplicates from one representative experiment of two. (C) The binding specificity of mAb 82.11 was determined by Western blot analysis using cell lysates collected from Vero cells infected with mock, DENV1 (Hawaii strain), DENV2 (NGC strain), DENV3 (H87 strain), DENV4 (H241 strain) or WNV (NY99 strain) . The NT 50 and dissociation constant (Kd) of mAb 82.11 are summarized on the right. The viron-capture ELISA of DENV1, DENV2 and DENV3 was performed using serial dilutions of mAb 82.11; the Kd was determined using the program GraphPad Prism 5.0. Data are means of duplicates from one representative experiment of two. ND, not done.

Article Snippet: To further validate the use of DENV1 virion- and VLP-capture in determining the [anti-E Abs], % anti-FL Abs and [anti-FL Abs] in this study, we used DENV3′ virion- and VLP-capture ELISA systems to determine the [anti-E Abs], % anti-FL Abs and [anti-FL Abs] in 14 DENV3 infection samples, and found a nice correlation with these three values determined by the two systems (DENV1 vs. DENV3: r = 0.91, P <0.0001; r = 0.78, P = 0.0009; and r = 0.74, P = 0.0027, respectively, two-tailed Spearman correlation test) ( ).

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation, Binding Assay, Western Blot, Infection

Journal: PLoS Neglected Tropical Diseases

Article Title: Analysis of Cross-Reactive Antibodies Recognizing the Fusion Loop of Envelope Protein and Correlation with Neutralizing Antibody Titers in Nicaraguan Dengue Cases

doi: 10.1371/journal.pntd.0002451

Figure Lengend Snippet: The current infecting serotype, previous infecting serotype(s) and “non-exposed serotypes” were determined as described in . [anti-FL Abs] and NT 50 against the current infecting serotype (A, E) and “non-exposed” serotypes (B to D, F to H) in patients with secondary DENV3 (A to D) and secondary DENV2 (E to H) infections.

Article Snippet: To further validate the use of DENV1 virion- and VLP-capture in determining the [anti-E Abs], % anti-FL Abs and [anti-FL Abs] in this study, we used DENV3′ virion- and VLP-capture ELISA systems to determine the [anti-E Abs], % anti-FL Abs and [anti-FL Abs] in 14 DENV3 infection samples, and found a nice correlation with these three values determined by the two systems (DENV1 vs. DENV3: r = 0.91, P <0.0001; r = 0.78, P = 0.0009; and r = 0.74, P = 0.0027, respectively, two-tailed Spearman correlation test) ( ).

Techniques: